Anti-ERGIC3 antibody (ab236723)
- Datasheet
- References
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Overview
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Product name
Anti-ERGIC3 antibody
See all ERGIC3 primary antibodies -
Description
Rabbit polyclonal to ERGIC3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Cynomolgus monkey, Orangutan
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Immunogen
Recombinant fragment corresponding to Human ERGIC3 aa 47-341.
Sequence:QYYLTTEVHPELYVDKSRGDKLKINIDVLFPHMPCAYLSIDAMDVAGEQQ LDVEHNLFKQRLDKDGIPVSSEAERHELGKVEVTVFDPDSLDPDRCESCY GAEAEDIKCCNTCEDVREAYRRRGWAFKNPDTIEQCRREGFSQKMQEQKN EGCQVYGFLEVNKVAGNFHFAPGKSFQQSHVHVHDLQSFGLDNINMTHYI QHLSFGEDYPGIVNPLDHTNVTAPQASMMFQYFVKVVPTVYMKVDGEVLR TNQFSVTRHEKVANGLLGDQGLPGVFVLYELSPMMVKLTEKHRSF
Database link: Q9Y282 -
Positive control
- WB: HepG2, HEK-293 and MCF7 whole cell lysates. IHC-P: Human rectum tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab236723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 43 kDa. | |
| IHC-P | 1/20 - 1/200. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Function
Possible role in transport between endoplasmic reticulum and Golgi. -
Sequence similarities
Belongs to the ERGIC family. -
Cellular localization
Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus > cis-Golgi network membrane. Endoplasmic reticulum membrane. Cycles between the endoplasmic reticulum and the Golgi. - Information by UniProt
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Database links
- Entrez Gene: 51614 Human
- Entrez Gene: 66366 Mouse
- SwissProt: Q9Y282 Human
- SwissProt: Q9CQE7 Mouse
- Unigene: 472558 Human
- Unigene: 141276 Mouse
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Alternative names
- 2310015B14Rik antibody
- AV318804 antibody
- C20orf47 antibody
see all
Images
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Western blot - Anti-ERGIC3 antibody (ab236723)All lanes : Anti-ERGIC3 antibody (ab236723) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC3 antibody (ab236723)Paraffin-embedded human rectum tissue stained for ERGIC3 using ab236723 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ERGIC3 antibody (ab236723)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ERGIC3 (green) using ab236723 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG(H+L).
References
ab236723 has not yet been referenced specifically in any publications.
