Product nameAnti-ERGIC3 antibody [EPR8141]
See all ERGIC3 primary antibodies
DescriptionRabbit monoclonal [EPR8141] to ERGIC3
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ERGIC3 aa 100-200. The exact sequence is proprietary.
- HepG2 cell lysate, MCF7 cell lysate, 293T cell lysate, K562 cell lysate, HeLa cells and cell lysate, Human brain tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.36 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
FunctionPossible role in transport between endoplasmic reticulum and Golgi.
Sequence similaritiesBelongs to the ERGIC family.
Cellular localizationEndoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus > cis-Golgi network membrane. Endoplasmic reticulum membrane. Cycles between the endoplasmic reticulum and the Golgi.
- Information by UniProt
- 2310015B14Rik antibody
- AV318804 antibody
- C20orf47 antibody
All lanes : Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : K562 cell lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
ab129179, at 1/100, staining ERGIC3 in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab129179, at 1/250, staining ERGIC3 in HeLa cells by Immunofluorescence.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129179 has not yet been referenced specifically in any publications.