Anti-ERK8 antibody (ab137619)
Key features and details
- Rabbit polyclonal to ERK8
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ERK8 antibody
See all ERK8 primary antibodies -
Description
Rabbit polyclonal to ERK8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ERK8 aa 1-245.
-
Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysate; A431 cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 60 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 60 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
In vitro, phosphorylates MBP. -
Tissue specificity
Widely expressed with a maximal expression in lung and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.
Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase. - Information by UniProt
-
Database links
- Entrez Gene: 225689 Human
- SwissProt: Q8TD08 Human
- Unigene: 493169 Human
-
Alternative names
- ERK 7 antibody
- ERK 8 antibody
- ERK-7 antibody
see all
Images
-
Anti-ERK8 antibody (ab137619) at 1/1000 dilution + HeLaS3 whole cell lysate at 30 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
ab137619 at 1/200 dilution staining ERK8 in methanol fixed A431 cells by Immunofluorescence. Lower image costained with Hoerchst 33342.
-
Immunohistochemical analysis of Human lung tissue, staining ERK8 in cytoplasm with ab137619, at 1/500 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab137619 has been referenced in 6 publications.
- Huang Q et al. Inhibition of ERK1/2 regulates cognitive function by decreasing expression levels of PSD-95 in the hippocampus of CIH rats. Eur J Neurosci 55:1471-1482 (2022). PubMed: 35243702
- Luan Y et al. REST-repressed lncRNA NPPA-AS1 regulates cervical cancer progression by modulating miR-302e/DKK1/Wnt/β-catenin signaling pathway. J Cell Biochem 122:16-28 (2021). PubMed: 32965043
- Zhang G et al. Tai Chi Improves Coronary Heart Disease Risk by Inactivating MAPK/ERK Pathway through Serum miR-126. Evid Based Complement Alternat Med 2020:4565438 (2020). PubMed: 32382289
- Su Z et al. Metastasis-associated gene MAPK15 promotes the migration and invasion of osteosarcoma cells via the c-Jun/MMPs pathway. Oncol Lett 20:99-112 (2020). PubMed: 32565938
- Li J et al. Thousand and one kinase 1 protects MCAO-induced cerebral ischemic stroke in rats by decreasing apoptosis and pro-inflammatory factors. Biosci Rep 39:N/A (2019). PubMed: 31652447
- Xu M et al. Linc00161 regulated the drug resistance of ovarian cancer by sponging microRNA-128 and modulating MAPK1. Mol Carcinog N/A:N/A (2018). PubMed: 30556928