Key features and details
- Rabbit polyclonal to ERK-8
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ERK-8 antibody
See all ERK-8 primary antibodies
DescriptionRabbit polyclonal to ERK-8
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human ERK-8 aa 1-245.
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysate; A431 cells
Previously labelled as MAPK15.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 60 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionIn vitro, phosphorylates MBP.
Tissue specificityWidely expressed with a maximal expression in lung and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.
Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.
- Information by UniProt
- ERK-7 antibody
- ERK-8 antibody
- ERK7 antibody
Anti-ERK-8 antibody (ab137619) at 1/1000 dilution + HeLaS3 whole cell lysate at 30 µg
Predicted band size: 60 kDa
7.5% SDS PAGE
ab137619 at 1/200 dilution staining ERK-8 in methanol fixed A431 cells by Immunofluorescence. Lower image costained with Hoerchst 33342.
Immunohistochemical analysis of Human lung tissue, staining ERK-8 in cytoplasm with ab137619, at 1/500 dilution.
ab137619 has been referenced in 2 publications.
- Li J et al. Thousand and one kinase 1 protects MCAO-induced cerebral ischemic stroke in rats by decreasing apoptosis and pro-inflammatory factors. Biosci Rep 39:N/A (2019). PubMed: 31652447
- Xu M et al. Linc00161 regulated the drug resistance of ovarian cancer by sponging microRNA-128 and modulating MAPK1. Mol Carcinog N/A:N/A (2018). PubMed: 30556928