Overview

  • Product name

  • Description

    Rabbit polyclonal to ERK-8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ERK-8 aa 395-444 (internal sequence).
    Sequence:

    HDPAEHESPRAAKNVPRQNSAPLLQTALLGNGERPPGAKEAPPLTLSLVK


    Database link: NP_620590

  • Positive control

    • MCF7 cell lysate
  • General notes

    Previously labelled as MAPK15. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 2% Sucrose
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    In vitro, phosphorylates MBP.
  • Tissue specificity

    Widely expressed with a maximal expression in lung and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.
    Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.
  • Information by UniProt
  • Database links

  • Alternative names

    • ERK-7 antibody
    • ERK-8 antibody
    • ERK7 antibody
    • ERK8 antibody
    • Extracellular regulated kinase 8 delta antibody
    • Extracellular signal regulated kinase 8 antibody
    • Extracellular signal-regulated kinase 7 antibody
    • Extracellular signal-regulated kinase 8 antibody
    • MAP kinase 15 antibody
    • MAPK 15 antibody
    • Mapk15 antibody
    • Mitogen activated protein kinase 15 antibody
    • Mitogen-activated protein kinase 15 antibody
    • MK15_HUMAN antibody
    see all

Images

  • Western blot analysis of untransfected HEK-293T (lane 1) and ERK-8 transfected HEK-293T (lane 2) lysates (50µg/lane) labeling ERK-8 with ab86275 at 1:2000. A HRP-conjugtaed anti-rabbit (1:1000) was used as the secondary antibody.

  • Sample Type : HA-ERK-8 transfected HeLa cells. Primary Antibody Dilution : 1:200. Secondary Antibody : Anti-rabbit-Alexa Fluor 555. Secondary Antibody Dilution : 1:800. Color/Signal Descriptions : A: Anti-HA-Alexa Fluor 488 B: ERK-8 C: DAPI D: Merge. Gene Name :ERK-8.

  • Anti-ERK-8 antibody (ab86275) at 1 µg/ml (5% skim milk/ PBS buffer) + MCF7 cell lysate at 10 µg with gel concentration: 12%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

References

ab86275 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab86275.
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