Anti-ERK1 + ERK2 antibody (ab17942)
- Datasheet
- References (83)
- Protocols
Overview
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Product nameAnti-ERK1 + ERK2 antibody
See all ERK1 + ERK2 primary antibodies -
DescriptionRabbit polyclonal to ERK1 + ERK2
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, ICC, ICC/IF, IHC-Fr, WBmore details
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Species reactivityReacts with: Mouse, Rat, Cow, Dog, Human, Culex pipiens
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Immunogen
Synthetic peptide corresponding to Human ERK1 + ERK2 aa 317-339 (C terminal).
Sequence:RIT VEEALAHPYL EQYYDPTDE
Database link: P27361 -
Positive control
- Mouse kidney tissue lysate - total protein (0 days) (ab7261) can be used as a positive control in WB. A431, PC12, and NIH3T3 cells.
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General notes
Please note that this is an intracellular epitope.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA
phosphate buffered saline without Mg2+ and
Ca2+. -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab17942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200. | |
| ICC | 1/200. | |
| ICC/IF | 1/200. | |
| IHC-Fr | 1/200. | |
| WB | 1/1000. Predicted molecular weight: 42-44 kDa. |
Target
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FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
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Post-translational
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 327672 Cow
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Omim: 176948 Human
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Alternative names
- ERK 1 antibody
- ERK 2 antibody
- ERK-2 antibody
see all
Images
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Western blot - Anti-ERK1 + ERK2 antibody (ab17942)This image is courtesy of an anonymous AbreviewAll lanes : Anti-ERK1 + ERK2 antibody (ab17942) at 1/1000 dilution
Lane 1 : Rat spinal cord tissue homogenate from animals that underwent Sham surgery
Lanes 2-3 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit antibody at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42-44 kDa
Observed band size: 42,44 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
The tissue was harvested seven days post surgery, sonicated with RIPA buffer and the protein estimate made by Lowry. A 10% SDS-PAGE gel was run for 1.5 hr at 100V and transfered to PVDF membrane for 1.5 hr at 274 mA. The blot was blocked with 5% BSA for 1 hour at 23°C. The primary antibody was incubated with the blot for 18 hours at 4°C. -
Immunohistochemistry (Frozen sections) - Anti-ERK1 + ERK2 antibody (ab17942)This image is courtesy of an anonymous Abreview.ab17942 staining ERK1 + ERK2 in Embryonic Stem Cell-induced Mouse Neurospheres by Immunohistochemistry (Frozen sections). Sections were formaldehyde-fixed prior to blocking in 10% serum for 30 minutes at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-Rabbit polyclonal antibody, diluted 1/1500 was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)Image courtesy of an anonymous Abreviewab17942 staining ERK1 + ERK2 in Human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 23°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 1 hourat 23°C. A HRP-conjugated GOat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunocytochemistry - Anti-ERK1 + ERK2 antibody (ab17942)This image is courtesy of an anonymous Abreview.ab17942 staining ERK1 + ERK2 in NIH3T3 Mouse Fibroblasts cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with 0.025% Triton-X in TBS and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal diluted to 1/1000 was used as the secondary antibody.
Blue channel - DAPI (nuclear staining)
Green channel - Alexa fluor 488 (B-Actin) -
Western blot - Anti-ERK1 + ERK2 antibody (ab17942)Image from PLoS One. 2014; 9(6): e99219. Fig3A, doi: 10.1371/journal.pone.0099219Western blot analysis of Mice retinas (40-50μg/lane) labelling with anti-ERK1/2 at 1:300 (ab17942) and mouse monoclonal anti-phosphorylated ERK1/2 at 1:300 (ab50011), in 5% nonfat milk in TBST overnight at 4ºC. HRP conjugated antibodies were used as the secondary antibodies.
Data is expressed as percentage change in phosphorylated ERK1/2 (p-ERK1/2) over total ERK1/2 (t-ERK1/2) calculated in control and diabetic mice maintained with and without Edaravone treatment
Results are expressed as mean±SD. Values obtained from Normal group are considered as 100%. *P<0.05, ***P<0.001 vs. Normal, #P<0.05 vs. Edaravone
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Western blot - Anti-ERK1 + ERK2 antibody (ab17942)Western Blot for ab17942.
Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated
with goat anti-rabbit IgG alkaline phosphatase.These data show that ab17942 ERK1&2 antibody allows the total amount of ERK1&2 to be measured.
Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat anti-ra
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)This image is courtesy of an anonymous Abreview
Protocols
References
This product has been referenced in:
- Zhao H et al. LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway. Oncol Lett 15:6839-6844 (2018). Read more (PubMed: 29731863) »
- Li Y et al. The anti-tumor effects of Mfn2 in breast cancer are dependent on promoter DNA methylation, the P21Ras motif and PKA phosphorylation site. Oncol Lett 15:8011-8018 (2018). Read more (PubMed: 29731912) »
