Overview

  • Product name
  • Description
    Rabbit polyclonal to ERK1 + ERK2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC, ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human, Culex pipiens
  • Immunogen

    Synthetic peptide corresponding to Human ERK1 + ERK2 aa 317-339 (C terminal).
    Sequence:

    RIT VEEALAHPYL EQYYDPTDE


    Database link: P27361

  • Positive control
    • Mouse kidney tissue lysate - total protein (0 days) (ab7261) can be used as a positive control in WB. A431, PC12, and NIH3T3 cells.
  • General notes

    Please note that this is an intracellular epitope.

Properties

Applications

Our Abpromise guarantee covers the use of ab17942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
ICC 1/200.
ICC/IF 1/200.
IHC-Fr 1/200.
WB 1/1000. Predicted molecular weight: 42-44 kDa.

Target

  • Function
    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK 1 antibody
    • ERK 2 antibody
    • ERK-2 antibody
    • ERK1 antibody
    • erk1/2 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p42-MAPK antibody
    • PRKM 2 antibody
    see all

Images

  • All lanes : Anti-ERK1 + ERK2 antibody (ab17942) at 1/1000 dilution

    Lane 1 : Rat spinal cord tissue homogenate from animals that underwent Sham surgery
    Lanes 2-3 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit antibody at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42-44 kDa
    Observed band size: 42,44 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    The tissue was harvested seven days post surgery, sonicated with RIPA buffer and the protein estimate made by Lowry. A 10% SDS-PAGE gel was run for 1.5 hr at 100V and transfered to PVDF membrane for 1.5 hr at 274 mA. The blot was blocked with 5% BSA for 1 hour at 23°C. The primary antibody was incubated with the blot for 18 hours at 4°C.

    See Abreview

  • ab17942 staining ERK1 + ERK2 in Embryonic Stem Cell-induced Mouse Neurospheres by Immunohistochemistry (Frozen sections). Sections were formaldehyde-fixed prior to blocking in 10% serum for 30 minutes at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-Rabbit polyclonal antibody, diluted 1/1500 was used as the secondary.

    See Abreview

  • ab17942 staining ERK1 + ERK2 in Human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 23°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 1 hourat 23°C. A HRP-conjugated GOat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab17942 staining ERK1 + ERK2 in NIH3T3 Mouse Fibroblasts cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with 0.025% Triton-X in TBS and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal diluted to 1/1000 was used as the secondary antibody.

    Blue channel - DAPI (nuclear staining)
    Green channel - Alexa fluor 488 (B-Actin)

    See Abreview

  • Western blot analysis of Mice retinas (40-50μg/lane) labelling with anti-ERK1/2 at 1:300 (ab17942) and mouse monoclonal anti-phosphorylated ERK1/2 at 1:300 (ab50011), in 5% nonfat milk in TBST overnight at 4ºC. HRP conjugated antibodies were used as the secondary antibodies.

    Data is expressed as percentage change in phosphorylated ERK1/2 (p-ERK1/2) over total ERK1/2 (t-ERK1/2) calculated in control and diabetic mice maintained with and without Edaravone treatment

    Results are expressed as mean±SD. Values obtained from Normal group are considered as 100%. *P<0.05, ***P<0.001 vs. Normal, #P<0.05 vs. Edaravone

  • Western Blot for ab17942.

    Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated
    with goat anti-rabbit IgG alkaline phosphatase.

    These data show that ab17942 ERK1&2 antibody allows the total amount of ERK1&2 to be measured.

     

    Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat anti-ra

  • ab17942 at 1/200 staining human (ab30203), rat (ab29480) and mouse (ab7261) kidney sections by IHC-P. The tissue sections were formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Li W  et al. Berberine suppresses IL-33-induced inflammatory responses in mast cells by inactivating NF-?B and p38 signaling. Int Immunopharmacol 66:82-90 (2019). Read more (PubMed: 30445310) »
  • Fang L  et al. Icariin inhibits cell proliferation, migration and invasion by down-regulation of microRNA-625-3p in thyroid cancer cells. Biomed Pharmacother 109:2456-2463 (2019). Read more (PubMed: 30551506) »
See all 92 Publications for this product

Customer reviews and Q&As

21-28 of 28 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (OE33 cells)
Total protein in input
20 µg
Immuno-precipitation step
Protein A/G
Specification
OE33 cells

Abcam user community

Verified customer

Submitted Oct 19 2010

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No

Dr. Sophie Pezet

Verified customer

Submitted Mar 03 2010

Application
Western blot
Sample
Rat Tissue lysate - whole (spinal cord homogenate)
Loading amount
20 µg
Specification
spinal cord homogenate
Treatment
sham or nerve transected
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 18 2008

Application
Western blot
Sample
Rat Cell lysate - whole cell (HAPI cell line)
Loading amount
400000 cells
Specification
HAPI cell line
Treatment
LPS 50 ng/mL or LPS 50 ng/mL+ UO 10 uM
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 10 2008

Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary Microglia)
Loading amount
400000 cells
Specification
Primary Microglia
Treatment
LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 10 2008

Application
Western blot
Sample
Mouse Cell lysate - whole cell (BV2 cell line)
Loading amount
400000 cells
Specification
BV2 cell line
Treatment
LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 10 2008

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (kidney)
Specification
kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer ph6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 07 2007

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS and 293T cell line)
Loading amount
30 µg
Specification
U2OS and 293T cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 02 2007

21-28 of 28 Abreviews or Q&A

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