The rat tissue homogenate used here was from animals that underwent either Sham surgery or L5 spinal nerve transection. The tissue was harvested seven days post surgery, sonicatedwith RIPA buffer, protein estimate made by Lowry and 20 ug loaded in each lane. A 10% SDS-PAGE gel was run for 1.5 hr at 100V and transfered to PVDF membrane for 1.5 hr at 274 mA.
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Submitted Jun 18 2008
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