Immunohistochemistry (Frozen sections) abreview for Anti-ERK1 + ERK2 antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Cell (Embryonic stem cells-induced neurosphere)
Specification
Embryonic stem cells-induced neurosphere
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1500

Additional data

Additional Notes
Works good. In particular experiment, stains only cytoplasm.
Cells are cryosectioned ESC-induced neurospheres.

Primary antibody was incubated overnight at 4C.

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Submitted Nov 23 2010

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