Immunocytochemistry/ Immunofluorescence abreview for Anti-ERK1 + ERK2 antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3 Mouse Fibroblasts)
Specification
NIH3T3 Mouse Fibroblasts
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in TBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/1000

Additional data

Additional Notes
Blue channel - DAPI (nuclear staining)
Green channel - Alexa fluor 488 (B-Actin)

Abcam user community

Verified customer

Submitted Jan 21 2011

Sign up