Western blot abreview for Anti-ERK1 + ERK2 antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (cortex)
Loading amount
25 µg
Specification
cortex
Gel Running Conditions
Reduced Denaturing (14% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer

Secondary antibody

Dilution
1/3000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 42 kDa

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Submitted May 31 2011

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