Western blot abreview for Anti-ERK1 + ERK2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (U2-OS)
Loading amount
25 µg
Specification
U2-OS
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey buffer

Secondary antibody

Name
Non-Abcam antibody was used: IR Dye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/10000

Detection

Detection method
Fluorescence
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 40, 45 kDa
Negative control
left without primary antibody

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Submitted Oct 18 2012

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