Western blot abreview for Anti-ERK1 + ERK2 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (stomach cancer)
Loading amount
30 µg
Specification
stomach cancer
Treatment
10uM WIN 55,212-2 for 24hrs
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: 2.5% NFDM, in 0.1% PBST

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 42, 44 kDa
Negative control
protein extract from normal cancer cell

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Submitted Apr 03 2013

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