Western blot abreview for Anti-ERK1 + ERK2 antibody

Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Culex pipiens Tissue lysate - whole (whole fly homogenates)
Specification
whole fly homogenates
Treatment
30 minutes after blood feeding with sheep blood
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000

Secondary antibody

Name
Dilution
1/50000

Detection

Detection method
ECL+
Bands
Specific: 50 kDa
Exposure
30 second(s)
Negative control
NONE
Positive control
30 minutes after blood feeding with sheep blood

Jiawen Xu

Verified customer

Submitted Aug 20 2013

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