Western blot abreview for Anti-ERK1 + ERK2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Hippocampus
Blocking step
LiCOR blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/5000

Secondary antibody

Dilution
1/50000
Name
Non-Abcam antibody was used: LiCOR IrDye680 donkey anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680

Detection

Detection method
Odyssey fluorescent imaging system
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 45 and 43 kDa
Positive control
Hippocampal samples

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Submitted Aug 27 2015

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