Western blot abreview for Anti-ERK1 + ERK2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic Fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20 µg
Specification
Mouse embryonic Fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk TBST
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG, HRP-linked
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 41, 44 kDa Non-specific: none kDa

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Submitted Oct 23 2018

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