Overview

  • Product name

  • Description

    Rabbit polyclonal to ERK1 + ERK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ERK 1/2 (Total) (internal sequence). The exact sequence is proprietary.
    Database link: P28482

  • Positive control

    • WB: K562 and NIH/3T3 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab196883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.

Target

  • Function

    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ERK 1 antibody
    • ERK 2 antibody
    • ERK-1 antibody
    • ERK-2 antibody
    • ERK1 antibody
    • erk1/2 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin-stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase 3 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • Microtubule-associated protein 2 kinase antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • Mitogen-activated protein kinase 3 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p41mapk antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • p44-ERK1 antibody
    • p44-MAPK antibody
    • p44ERK1 antibody
    • p44MAPK antibody
    • PRKM 2 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • PRKM3 antibody
    • protein tyrosine kinase ERK2 antibody
    see all

Images

  • All lanes : Anti-ERK1 + ERK2 antibody (ab196883) at 1/500 dilution

    Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell extract
    Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell extract with synthesized peptide

    Predicted band size: 41 kDa

  • All lanes : Anti-ERK1 + ERK2 antibody (ab196883) at 1/500 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extract with synthesized peptide
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extract

    Predicted band size: 41 kDa

References

This product has been referenced in:

  • Fan L  et al. Sevoflurane inhibits the migration and invasion of colorectal cancer cells through regulating ERK/MMP-9 pathway by up-regulating miR-203. Eur J Pharmacol 850:43-52 (2019). Read more (PubMed: 30685432) »
  • Zhang L  et al. FLOT1 promotes tumor development, induces epithelial-mesenchymal transition, and modulates the cell cycle by regulating the Erk/Akt signaling pathway in lung adenocarcinoma. Thorac Cancer 10:909-917 (2019). Read more (PubMed: 30838797) »
See all 25 Publications for this product

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