Recombinant Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
![Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](https://www.abcam.com/ps/products/184/ab184699/Images/ab184699-239197-anti-erk1-erk2-antibody-epr17526-western-blot.jpg "Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17526] to ERK1 + ERK2
- Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ERK1 + ERK2 antibody [EPR17526]
See all ERK1 + ERK2 primary antibodies -
Description
Rabbit monoclonal [EPR17526] to ERK1 + ERK2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human ERK1 full length recombinant protein; Human ERK2 full length recombinant protein; A431, Jurkat, HeLa, HepG2, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, heart, kidney and spleen lysates. ICC/IF: HeLa cells. Flow Cyt (intra): A431 cells. IP. PC-12 whole cell extract.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17526 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-ERK1 + ERK2 antibody [EPR17526] (ab208564)
- Alexa Fluor® 647 Anti-ERK1 + ERK2 antibody [EPR17526] (ab208881)
- HRP Anti-ERK1 + ERK2 antibody [EPR17526] (ab209321)
- Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)
- Alexa Fluor® 594 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210460)
- Alexa Fluor® 555 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210659)
- PE Anti-ERK1 + ERK2 antibody [EPR17526] (ab212153)
- Anti-ERK1 + ERK2 antibody [EPR17526] - BSA and Azide free (ab218017)
- Anti-ERK2 antibody [1B3B9] (ab231085)
- Anti-ERK2 antibody [1B3B9] - BSA and Azide free (ab269567)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/440.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP |
1/70.
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| ICC/IF | (1) |
1/250.
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| WB |
1/10000. Detects a band of approximately 44, 42 kDa (predicted molecular weight: 43, 41 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
1/440. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP
1/70. |
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ICC/IF
1/250. |
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WB
1/10000. Detects a band of approximately 44, 42 kDa (predicted molecular weight: 43, 41 kDa). |
Target
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Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Omim: 176948 Human
- Omim: 601795 Human
see all -
Form
Mainly expressed in the cytoplasm and only localizes to the nucleus with treatment. -
Alternative names
- ERK 1 antibody
- ERK 2 antibody
- ERK-1 antibody
see all
Images
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Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Mouse heart lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse spleen lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lane 7 : Rat kidney lysates
Lane 8 : Rat spleen lysates
Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43, 41 kDa
Observed band size: 44, 42 kDa why is the actual band size different from the predicted?44kDa band represents ERK1. 42kDa band represents ERK2.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](https://www.abcam.com/ps/products/184/ab184699/Images/ab184699-388728-anti-erk1-erk2-antibody-epr17526-western-blot-hela-lysates.jpg)
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MAPK1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 43, 41 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?Lanes 1-2: Merged signal (red and green). Green - ab184699 observed at 44 kDa. Red - loading control ab8245 observed at 37 kDa.
ab184699 Anti-ERK1 + ERK2 antibody [EPR17526] was shown to specifically react with ERK2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265052 (knockout cell lysate ab257525) was used. Wild-type and ERK2 knockout samples were subjected to SDS-PAGE. ab184699 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](https://www.abcam.com/ps/products/184/ab184699/Images/ab184699-284667-anti-erk1-erk2-antibody-epr17526-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)This image is courtesy of an Abreview submitted by Kirk Mcmanus.Ab184699 staining ERK1 + ERK2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) was used as the secondary antibody.
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![Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](https://www.abcam.com/ps/products/184/ab184699/Images/ab184699-239201-anti-erk1-erk2-antibody-epr17526-western-blot.jpg)
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution + Recombinant Human ERK1 protein (ab43623) (ab43623) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43, 41 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?Recombinant full length ERK1 protein (ab43623) contains aa1-379.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](https://www.abcam.com/ps/products/184/ab184699/Images/ab184699-239193-anti-erk1-erk2-antibody-epr17526-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ERK1 + ERK2 with ab184699 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing both nuclear and cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab184699 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](https://www.abcam.com/ps/products/184/ab184699/Images/ab184699-239200-anti-erk1-erk2-antibody-epr17526-western-blot.jpg)
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution +Recombinant Human ERK2 protein (ab43625) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43, 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?Recombinant full length ERK2 protein (ab43625) contains aa1-360.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](https://www.abcam.com/ps/products/184/ab184699/Images/ab184699-239199-anti-erk1-erk2-antibody-epr17526-western-blot.jpg)
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/50000 dilution
Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 4 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysates
Lane 5 : Human fetal brain lysates
Lane 6 : Human fetal heart lysates
Lane 7 : Human fetal kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43, 41 kDa
Observed band size: 42,44 kDa why is the actual band size different from the predicted?44kDa band represents ERK1. 42kDa band represents ERK2.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](https://www.abcam.com/ps/products/184/ab184699/Images/ab184699-239198-anti-erk1-erk2-antibody-epr17526-western-blot.jpg)
Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal heart lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 43, 41 kDa
Observed band size: 42,44 kDa why is the actual band size different from the predicted?44kDa band represents ERK1. 42kDa band represents ERK2.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry (Intracellular) - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](https://www.abcam.com/ps/products/184/ab184699/Images/ab184699-239192-anti-erk1-erk2-antibody-epr17526-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)Intracellular flow cytometric analysis of A431 (Human epidermoid carcinoma) cells labeling ERK1 + ERK2 with ab184699 at 1/440 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](https://www.abcam.com/ps/products/184/ab184699/Images/ab184699-239194-anti-erk1-erk2-antibody-epr17526-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)ERK1 + ERK2 were immunoprecipitated from 1mg of PC-12 (Rat adrenal gland pheochromocytoma) whole cell extract with ab184699 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab184699 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: PC-12 whole cell extract. Lane 2: PBS instead of PC-12 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.44kDa band represents ERK1. 42kDa band represents ERK2.
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![Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)](https://www.abcam.com/ps/products/184/ab184699/Images/ab184699-11-benefits-of-recombinant-antibodies.png)
Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (121)
ab184699 has been referenced in 121 publications.
- Shi L et al. Spaceflight and simulated microgravity suppresses macrophage development via altered RAS/ERK/NF?B and metabolic pathways. Cell Mol Immunol 18:1489-1502 (2021). PubMed: 31900461
- He XL et al. Solid lipid nanoparticles loading with curcumin and dexanabinol to treat major depressive disorder. Neural Regen Res 16:537-542 (2021). PubMed: 32985484
- Wang X et al. C-C chemokine receptor 5 signaling contributes to cardiac remodeling and dysfunction under pressure overload. Mol Med Rep 23:N/A (2021). PubMed: 33200795
- Fei J et al. Inhibition of SNCG suppresses the proliferation of lung cancer cells induced by high glucose. Mol Med Rep 23:N/A (2021). PubMed: 33313952
- Hou TY et al. JNK and p38 Inhibitors Prevent Transforming Growth Factor-ß1-Induced Myofibroblast Transdifferentiation in Human Graves' Orbital Fibroblasts. Int J Mol Sci 22:N/A (2021). PubMed: 33799469