Product nameAnti-ERK1 + ERK2 antibody [EPR17526] (Phycoerythrin)
See all ERK1 + ERK2 primary antibodies
DescriptionRabbit monoclonal [EPR17526] to ERK1 + ERK2 (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P28482
- Flow Cyt: A431 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
- Anti-ERK1 + ERK2 antibody [EPR17526] (Alexa Fluor® 488) (ab208564)
- Anti-ERK1 + ERK2 antibody [EPR17526] (Alexa Fluor® 647) (ab208881)
- Anti-ERK1 + ERK2 antibody [EPR17526] (HRP) (ab209321)
- Anti-ERK1 + ERK2 antibody [EPR17526] (Alexa Fluor® 405) (ab210044)
- Anti-ERK1 + ERK2 antibody [EPR17526] (Alexa Fluor® 594) (ab210460)
- Anti-ERK1 + ERK2 antibody [EPR17526] (Alexa Fluor® 555) (ab210659)
- Anti-ERK1 + ERK2 antibody [EPR17526] - BSA and Azide free (ab218017)
Our Abpromise guarantee covers the use of ab212153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF.
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
- Information by UniProt
- ERK 1 antibody
- ERK 2 antibody
- ERK-2 antibody
Overlay histogram showing A431 cells stained with ab212153 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab212153, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
ab212153 has not yet been referenced specifically in any publications.