Western blot abreview for Anti-ERK1 + ERK2 [ERK-7D8] antibody

Excellent
Abreviews
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Human Cell lysate - whole cell (Human Granulosa cell line)
Specification
Human Granulosa cell line
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer + 0.2% Tween
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: RDye 680LT Goat anti-Mouse IgG1 (H + L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 680
Dilution
1/25000

Detection

Detection method
Infrared
Bands
Specific: 44 kDa
Exposure
4 minute(s) and 0 second(s)

Additional data

Additional Notes
Picture shows WB on two replicates of human granulosa cell line. This antibody works very well with the infrared technology tested.

Dr. Francesco Elia Marino

Verified customer

Submitted Jun 02 2014

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