Immunocytochemistry/ Immunofluorescence abreview for Anti-ERK1 + ERK2 [ERK-7D8] antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Sample
Human Cell (Human granulosa cell Line)
Specification
Human granulosa cell Line
Permeabilization
Yes - Ethanol and Triton
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit IgG (H + L), 0.5mg
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 800CW
Dilution
1/500

Additional data

Additional Notes
Picture shows (left to right) 4 replicates Negative Control, Media and Treatment.

Dr. Francesco Elia Marino

Verified customer

Submitted Jun 19 2014

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