Key features and details
- Rabbit monoclonal [SP327] to ERK1 + ERK2 (phospho T202 + Y204)
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327]
See all ERK1 + ERK2 primary antibodies
DescriptionRabbit monoclonal [SP327] to ERK1 + ERK2 (phospho T202 + Y204)
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human ERK1 + ERK2 (phospho T202 + Y204).
Database link: P27361
- WB: A431 + EGF cell lysates. IHC-P: Human breast ductal carcinoma, bladder transitional cell carcinoma and breast tissues. Flow Cyt: A431 + EGF cells.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/400. Predicted molecular weight: 42,44 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
- Information by UniProt
- ERK 1 antibody
- ERK 2 antibody
- ERK-1 antibody
All lanes : Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500) at 1/400 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) + EGF cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Predicted band size: 42,44 kDa
Paraffin-embedded human breast ductal carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.
Flow cytometric analysis of ab223500 in A431 (human epidermoid carcinoma cell line) cells + EGF (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).
Paraffin-embedded human breast tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human bladder transitional cell carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab223500 has been referenced in 14 publications.
- Ma Y et al. Fli-1 Activation through Targeted Promoter Activity Regulation Using a Novel 3', 5'-diprenylated Chalcone Inhibits Growth and Metastasis of Prostate Cancer Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32210104
- Cao X et al. Y-box binding protein 1 regulates ox-LDL mediated inflammatory responses and lipid uptake in macrophages. Free Radic Biol Med 141:10-20 (2019). PubMed: 31153975
- He C et al. LncRNA UCA1 acts as a sponge of miR-204 to up-regulate CXCR4 expression and promote prostate cancer progression. Biosci Rep 39:N/A (2019). PubMed: 30940776
- Huang H et al. Downregulation of Keap1 contributes to poor prognosis and Axitinib resistance of renal cell carcinoma via upregulation of Nrf2 expression. Int J Mol Med 43:2044-2054 (2019). PubMed: 30896860
- Xu J et al. MAT2B expression correlates with poor prognosis in triple-negative breast cancer. Cancer Manag Res 11:5501-5511 (2019). PubMed: 31354356