Product nameAnti-ERK1 antibody [12D11]
See all ERK1 primary antibodies
DescriptionMouse monoclonal [12D11] to ERK1
Tested applicationsSuitable for: WB, ICC/IF, IHC-P, ChIPmore details
Species reactivityReacts with: Mouse, Human, Non human primates
Recombinant full length Human ERK1 expressed in bacteria.
- 293, U2OS, K562, A549, HeLa, Jurkat, MCF7, HepG2, MEF, NIH 3T3, C2C12 and COS7 cell lysates; HeLa cells; Human colon cancer tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab119357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 43 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ChIP||Use at an assay dependent concentration.
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
- Information by UniProt
- ERK 1 antibody
- ERK-1 antibody
- ERK1 antibody
All lanes : Anti-ERK1 antibody [12D11] (ab119357) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : U2OS cell lysate
Lane 3 : K562 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : MCF7 cell lysate
Lane 8 : HepG2 cell lysate
Lane 9 : MEF cell lysate
Lane 10 : NIH 3T3 cell lysate
Lane 11 : C2C12 cell lysate
Lane 12 : COS7 cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-mouse-HRP at 1/25000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa
4-20% Tris-HCl polyacrylamide gel.
ab119357, at 1/100 dilution, staining ERK1 in untreated and siRNA knockdown treated HeLa cells (Formalin fixed and permeabilized with 0.1% Triton X-100) by Immunoflourescence followed by DyLight 549 goat-anti-mouse secondary antibody at a dilution of 1/400.
Nuclei (blue) were stained with Hoechst 33342 dye.
ab119357, at 1/100 dilution, staining ERK1 in deparaffinized Human colon cancer tissue by Immunohistochemistry using a goat anti-mouse HRP secondary antibody at a dilution of 1/500 and colorimetric detection using metal enhanced DAB.
Note: strong cytoplasmic localization of ERK1.
ab119357 has not yet been referenced specifically in any publications.