Product nameAnti-ERK1 antibody
See all ERK1 primary antibodies
DescriptionRabbit polyclonal to ERK1
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide aa 329-379. The exact sequence is proprietary. NP_002737.2
Database link: P27361
- IHC-P:human breast carcinoma tissue; IP: HeLa whole cell lysate; WB: HeLa and Hek-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500.|
|WB||1/2000 - 1/10000.|
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
- Information by UniProt
- ERK 1 antibody
- ERK-1 antibody
- ERK1 antibody
All lanes : Anti-ERK1 antibody (ab264269) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Hek293-T whole cell lysate at 50 µg
Detected with chemilimuniscence with exposure time of 30 seconds.
ERK1was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264269 at 3 μg per reaction. Western blot was performed on the immunoprecipitates using ab264269 at 1 μg/ml.
Lane 1: ab264269 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 3 seconds.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for ERK1 using ab264269 at 1/200 dilution in immunohistochemical analysis. DAB staining.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264269 has not yet been referenced specifically in any publications.