Product nameAnti-ERK1 + ERK2 antibody
See all ERK1 + ERK2 primary antibodies
DescriptionRabbit polyclonal to ERK1 + ERK2
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Predicted to work with: Sheep, Chicken, Hamster, Xenopus laevis, Drosophila melanogaster
Synthetic peptide corresponding to a region of Rat ERK1 + ERK2.
- Human A431 fibroblast cell lysate; Human breast cancer tissue; U251 cells.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab115799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 42, 44 kDa (predicted molecular weight: 41 kDa).|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 10 µg/ml.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
- Information by UniProt
- ERK 1 antibody
- ERK 2 antibody
- ERK-1 antibody
Anti-ERK1 + ERK2 antibody (ab115799) at 1/1000 dilution + Human A431 fibroblast cell lysate
Predicted band size: 41 kDa
ab115799 at 1/50 dilution staining ERK1 + ERK2 in Paraffin-embedded Human breast cancer tissue by Immunohistochemistry.
Flow cytometry analysis of U251 cells using ab115799 at 10ug/ml (right) and control rabbit IgG (left).
This product has been referenced in:
- Han B et al. Cimifugin Inhibits Inflammatory Responses of RAW264.7 Cells Induced by Lipopolysaccharide. Med Sci Monit 25:409-417 (2019). Read more (PubMed: 30638197) »
- Zhu AD et al. lncRNA-ATB promotes viability, migration, and angiogenesis in human microvascular endothelial cells by sponging microRNA-195. J Cell Biochem N/A:N/A (2019). Read more (PubMed: 30983015) »