Key features and details
- Rabbit polyclonal to ERK1 + ERK2
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-ERK1 + ERK2 antibody
See all ERK1 + ERK2 primary antibodies
DescriptionRabbit polyclonal to ERK1 + ERK2
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Sheep, Chicken, Cow, Xenopus laevis, Drosophila melanogaster
A 35 residue synthetic peptide, conjugated to KLH, corresponding to Rat ERK1 with CGG spacer group (Uniprot: P28482H).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab166847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
- Information by UniProt
- ERK 1 antibody
- ERK 2 antibody
- ERK-1 antibody
Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human spleen tissue labeling ERK1 + ERK2 with ab166847 at 1/100 dilution.
Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human skin tissue labeling ERK1 + ERK2 with ab166847 at 1/100 dilution.
Immunocytochemistry/Immunofluorescence analysis of Mouse back skin tissue labeling ERK1 + ERK2 with ab166847.
ab166847 has been referenced in 1 publication.
- Zheng XF et al. MicroRNA-192 acts as a tumor suppressor in colon cancer and simvastatin activates miR-192 to inhibit cancer cell growth. Mol Med Rep 19:1753-1760 (2019). PubMed: 30628692