Asynchronous cells were left untreated, or treated with either PBDu (1uM 30min) or U0126 (10uM 1 h), paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Following PDBu treatment, this antibody appears to become highly enriched within nuclei versus a more diffuse and equally distributed pattern (cytoplasm + nucleus) in untreated cells. Following U0126 treatment, this antibody does not appear to change localization and appears similar to the untreated condition.
Dr. Kirk Mcmanus
Submitted Jul 26 2017
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