Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody (ab128159)
Key features and details
- Rabbit polyclonal to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody -
Description
Rabbit polyclonal to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
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Positive control
- HeLa whole cell lysate, mock treated or treated with TPA
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 41, 43 kDa.
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 41, 43 kDa. |
IP
Use at an assay dependent concentration. |
Target
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Cellular localization
ERK2: Nucleus. -
Database links
- Entrez Gene: 373953 Chicken
- Entrez Gene: 327672 Cow
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 100153927 Pig
- Entrez Gene: 116590 Rat
see all
Images
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All lanes : Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody (ab128159) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate, mock treated
Lane 2 : HeLa whole cell lysate, TPA treated
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 41, 43 kDa
Exposure time: 30 seconds -
ab128159 at 1 µg/ml staining ERK1 + ERK2 (phospho T202 + Y204 + T185 + Y187) in HeLa whole cell lysate immunoprecipitated using a Rabbit anti-ERK1 antibody (1 mg/IP; 20% of IP loaded/lane).
Lane 1: mock treated.
Lanes 2 and 3: TPA treated.
Lane 3: normal goat IgG.
Predicted band size: 41 kDa.
Exposure time: 30 seconds.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab128159 has not yet been referenced specifically in any publications.