Product nameAnti-ERK1 (phospho T202 + Y204) antibody
See all ERK1 primary antibodies
DescriptionRabbit polyclonal to ERK1 (phospho T202 + Y204)
Specificityab24157 reacts with Phospho-Thr202/Thr204 ERK/MAPK protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human, Xenopus laevis
Synthetic peptide corresponding to Rat ERK1 (internal sequence) (phospho T202 + Y204).
- rat brain lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab24157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 43 kDa).|
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
- Information by UniProt
- ERK 1 antibody
- ERK antibody
- ERK-1 antibody
Anti-ERK1 (phospho T202 + Y204) antibody (ab24157) at 1/1000 dilution + Rat brain lysate
Predicted band size: 43 kDa
Observed band size: 42-44 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Han M et al. Hispidulin inhibits hepatocellular carcinoma growth and metastasis through AMPK and ERK signaling mediated activation of PPAR?. Biomed Pharmacother 103:272-283 (2018). WB ; Human . Read more (PubMed: 29656183) »
- Yan N et al. Promotion of anoxia-reoxygenation-induced inflammation and permeability enhancement by nicotinamide phosphoribosyltransferase-activated MAPK signaling in human umbilical vein endothelial cells. Exp Ther Med 14:4595-4601 (2017). Read more (PubMed: 29104667) »