Key features and details
- Rabbit polyclonal to ERK1 (phospho Y204)
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ERK1 (phospho Y204) antibody
See all ERK1 primary antibodies
DescriptionRabbit polyclonal to ERK1 (phospho Y204)
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ERK1 (phospho Y204). The exact sequence is proprietary. Phospho specific peptide corresponding to residues surrounding Tyrosine 204 of Human ERK1.
Database link: P27361
- SK-BR-3 cell extract (treated by EGF + Insulin and untreated); Human breast carcinoma tissue; HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/200.|
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 43 kDa.|
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
- Information by UniProt
- ERK 1 antibody
- ERK-1 antibody
- ERK1 antibody
All lanes : Anti-ERK1 (phospho Y204) antibody (ab194770) at 1/500 dilution
Lane 1 : SK-BR-3 cell extract untreated
Lane 2 : SK-BR-3 cell extract treated by EGF + Insulin
Predicted band size: 43 kDa
Immunofluorescent analysis of methanol-fixed HeLa cells labeling ERK1 (phospho Y204) with ab194770 at 1/100.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ERK1 (phospho Y204) with ab194770 at 1/50.
ab194770 has not yet been referenced specifically in any publications.