Overview

  • Product name
    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody
    See all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies
  • Description
    Rabbit polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human, Culex pipiens
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.
    (Peptide available as ab5313, ab5354, ab5255)

  • Positive control
    • PC12 cells +/- Sorbitol.

Properties

Applications

Our Abpromise guarantee covers the use of ab4819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 44,42 kDa.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Images

  • Peptide Competition: Extracts prepared from PC12 cells stimulated with NGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4819 antibody for two hours at room temperature, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4819 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

    Peptide Competition: Extracts prepared from PC12 cells stimulated with NGF were resolved by SDS-PAGE on
  • Figure shows immunohistochemical localization of active ab4819 in sections from rac1 expressing cardiomyopathic animals displaying the dilated phenotype. Green = p-ERK1/2, Red = nuclei, and Blue = alpha-actinin.

References

This product has been referenced in:
  • Fa Z  et al. MicroRNA-150 suppresses the growth and malignant behavior of papillary thyroid carcinoma cells via downregulation of MUC4. Exp Ther Med 16:45-52 (2018). Read more (PubMed: 29896226) »
  • Kole D  et al. High molecular weight FGF2 isoforms demonstrate canonical receptor-mediated activity and support human embryonic stem cell self-renewal. Stem Cell Res 21:106-116 (2017). Read more (PubMed: 28433654) »
See all 20 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Mouse Neuroblastoma (Neuro2A))
Specification
Mouse Neuroblastoma (Neuro2A)
Treatment
Synthetic Spiro analogues
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Abcam user community

Verified customer

Submitted Sep 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Culex pipiens Tissue lysate - whole (whole fly homogenates)
Specification
whole fly homogenates
Treatment
30 minutes after feeding with sheep blood
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Jiawen Xu

Verified customer

Submitted Aug 20 2013

Answer

Thank you for contacting us.

I am sorry to that the antibody seems not tobe working as expected.

As discussed over the phone, please send me some of your WB images and additional protocol information, so that I can pass this on to the lab for additional suggestions and feedback.

I look forward to hear back from you and assist you further.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

I am happy to help! Please feel free to contact me if you need anything further.

Read More

Answer

I am sorry this product did not contain the volume as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question
Answer

Thank you for your phone call.


DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab4819 in termites. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for termitesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.


The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Termite Tissue lysate - whole (termite)
Loading amount
40 µg
Specification
termite
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 23°C

Mr. Chris Mattison

Verified customer

Submitted Apr 09 2012

Question
Answer

DISCOUNT CODE: *** Expiration date: March 30th, 2012 I am very pleased to hear you would like to accept our offer and test ab4819 in termite. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for termite and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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Application
Western blot
Sample
Human Cell lysate - whole cell (prostate)
Loading amount
25 µg
Specification
prostate
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jan 03 2007

Answer

Thank you for contacting us with your query. There is only a small difference between these two antibodies if you are going to make a choice between the two. They will both recognise phosphorylated ERK1 and ERK2. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating their activity . Ab4819 recognises phosphorylation on the T185 and T202 residues Ab16869 recognises phosphorylation on T202/T183 and Y204/185 residues. These phosphorylated residues are common to ERK 1 and 2. Both the antibodies are raised against synthetic peptide. I hope this helps! Please get back to me if there is anything else I can help you with. With thanks.

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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