Product nameAnti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody
See all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies
DescriptionRabbit polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
Tested applicationsSuitable for: WB, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Rat, Human, Culex pipiens
Predicted to work with: a wide range of other species
- PC12 cells +/- Sorbitol.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
BSA is IgG and protease free
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the sites of phosphorylation to remove antibody that is reactive with non-phosphorylated ERK 1 + 2. The final product is generated by affinity chromatography using an ERK 1 + 2-derived peptide that is phosphorylated at threonine 202/185 and tyrosine 204/187, respectively, within the activation loop.
Our Abpromise guarantee covers the use of ab4819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 44,42 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 10µl for 106 cells.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Peptide Competition: Extracts prepared from PC12 cells stimulated with NGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50Peptide Competition: Extracts prepared from PC12 cells stimulated with NGF were resolved by SDS-PAGE on
µg/mL ab4819 antibody for two hours at room temperature, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4819 blocks the antibody signal, thereby demonstrating the specificity of the antibody.
Figure shows immunohistochemical localization of active ab4819 in sections from rac1 expressing cardiomyopathic animals displaying the dilated phenotype. Green = p-ERK1/2, Red = nuclei, and Blue = alpha-actinin.
This product has been referenced in:
- Li W et al. Berberine suppresses IL-33-induced inflammatory responses in mast cells by inactivating NF-?B and p38 signaling. Int Immunopharmacol 66:82-90 (2019). Read more (PubMed: 30445310) »
- Ursoli Ferreira F et al. Endothelial Cells Tissue-Specific Origins Affects Their Responsiveness to TGF-ß2 during Endothelial-to-Mesenchymal Transition. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30678183) »