Key features and details
- Rabbit monoclonal [G15-B] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [G15-B]
See all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies
DescriptionRabbit monoclonal [G15-B] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). Including the conserved pT-E-pY motif
- PC12 cell lysates (treated with EGF, PMA or EGF); HEK293 cells treated with hydrogen peroxide.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 0.32% Tris HCl, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.
Our Abpromise guarantee covers the use of ab136926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Predicted molecular weight: 41, 43 kDa.|
All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [G15-B] (ab136926) at 1/5000 dilution
Lane 1 : PC12 cell lysate - untreated
Lane 2 : PC12 cell lysate - treated with EGF-100ng/ml (5min) in serum-free DMEM
Lane 3 : PC12 cell lysate - treated with PMA-100nM (30min) in serum-free DMEM
Lane 4 : PC12 cell lysate treated with EGF-100ng/ml (5min) with 10% FBS in DMEM
Lysates/proteins at 50 µg per lane.
Predicted band size: 41, 43 kDa
Immunocytochemical analysis of HEK293 cells labelling phospho-ERK1 + ERK2 with ab136926 at 1/100 dilution.
Immunocytochemical analysis of HEK293 cells (hydrogen peroxide treated) labelling phospho-ERK1 + ERK2 with ab136926 at 1/100 dilution.
ab136926 has been referenced in 3 publications.
- He X et al. HOXB7 overexpression promotes cell proliferation and correlates with poor prognosis in gastric cancer patients by inducing expression of both AKT and MARKs. Oncotarget 8:1247-1261 (2017). WB ; Human . PubMed: 27901487
- Yu N et al. Serum amyloid A, an acute phase protein, stimulates proliferative and proinflammatory responses of keratinocytes. Cell Prolif 50:N/A (2017). PubMed: 27910163
- Venegas LA et al. A comparison of phosphospecific affinity reagents reveals the utility of recombinant Forkhead-associated domains in recognizing phosphothreonine-containing peptides. N Biotechnol 33:537-43 (2016). PubMed: 26772725