Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) [MAPK-YT] antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako REALPeroxidase-Blocking Solution S2023 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Antigen retrieval step
Heat mediated
Sample
Zebrafish Tissue sections (Liver, dysplatic cells)
Specification
Liver, dysplatic cells
Permeabilization
No
Fixative
Paraformaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 28°C · Diluent: Dako,Antibody Diluent: #3022

Secondary antibody

Name
Non-Abcam antibody was used: ChemMate™ DAKO EnVision™ Detection Kit, Peroxidase
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Seems to work. But not highly specific. Recommended dilution factor 1: 500

Dr. Inhye Jung

Verified customer

Submitted Jul 17 2014

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