Immunohistochemistry (Frozen sections) abreview for Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT]

Poor
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Aceton methanol

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: jackson
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein

Additional data

Abcam response
Thank you for participating to our AbTrial program by testing ab50011 in IHC-Fr, an untested application. I suggest to test this antibody using an alternative fixative such as formaldehyde. It worked well in immunocytochemistry.

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Verified customer

Submitted Feb 06 2017

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