Immunohistochemistry (Frozen sections) abreview for Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT]

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Xenopus laevis Tissue sections (Tadpole limb)
Permeabilization
Yes - 1% Triton X-100
Specification
Tadpole limb
Blocking step
Roche blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 555 anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555

Additional data

Additional Notes
ABTRIAL-EVQCH
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

Gufa Lin

Verified customer

Submitted Sep 12 2017

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