Western blot abreview for Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT]

Excellent
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12 cell)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
PC12 cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-Mouse IgG, HRP-linked Antibody
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 42 kDa

Miss. Ji Yeon Lee

Verified customer

Submitted Jan 25 2019

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