Overview

  • Product name

    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody
    See all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies
  • Description

    Rabbit polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to amino acids surrounding phosphorylated threonine 202 and tyrosine 204 of Human ERK1 (NP_002737.2) and phosphorylated threonine 185 and tyrosine 187 of Human ERK2 (NP_620407.1).

  • Positive control

    • HeLa whole cell lysate, mock treated or treated with TPA

Properties

Applications

Our Abpromise guarantee covers the use of ab128159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 41, 43 kDa.
IP Use at an assay dependent concentration.

Images

  • All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab128159) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate, mock treated
    Lane 2 : HeLa whole cell lysate, TPA treated

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 41, 43 kDa


    Exposure time: 30 seconds
  • ab128159 at 1 µg/ml staining ERK1 + ERK2 (phospho T202 + Y204 + T185 + Y187) in HeLa whole cell lysate immunoprecipitated using a Rabbit anti-ERK1 antibody (1 mg/IP; 20% of IP loaded/lane).
    Lane 1: mock treated.
    Lanes 2 and 3: TPA treated.
    Lane 3: normal goat IgG.
    Predicted band size: 41 kDa.
    Exposure time: 30 seconds.

References

ab128159 has not yet been referenced specifically in any publications.

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