Key features and details
- Goat polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody
See all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies
DescriptionGoat polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide, corresponding to a region surrounding threonine 202 and tyrosine 204 of Human ERK1 (NP_002737.2) and threonine 185 and tyrosine 187 of Human ERK2 (NP_620407.1) when the threonine and tyrosine residues are phosphorylated.
- HeLa whole cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab128177 is affinity purified using an epitope specific to Phospho ERK1 + ERK2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab128177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
Detection of ERK1 + ERK2 by Western Blot of Immunprecipitate.
anti-ERK1 (lanes 1,2) and anti-ERK2 (lanes 3,4) at at 1µg/ml and anti-ERK1 and ERK2 antibody mixture (lanes 3,4,5,6) at 1µg/ml each staining ERK1 + ERK2 in HeLa whole cell lysate immunoprecipitated using ab128177 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
HeLa cells that were mock treated (-) or treated with TPA (+).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab128177 has not yet been referenced specifically in any publications.