Overview

  • Product name

    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [G15-B]
    See all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies
  • Description

    Rabbit monoclonal [G15-B] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, ELISAmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). Including the conserved pT-E-pY motif

  • Positive control

    • PC12 cell lysates (treated with EGF, PMA or EGF); HEK293 cells treated with hydrogen peroxide.

Properties

Applications

Our Abpromise guarantee covers the use of ab136926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/5000. Predicted molecular weight: 41, 43 kDa.
IP Use at an assay dependent concentration.
ELISA 1/20000 - 1/100000.

Images

  • All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [G15-B] (ab136926) at 1/5000 dilution

    Lane 1 : PC12 cell lysate - untreated
    Lane 2 : PC12 cell lysate - treated with EGF-100ng/ml (5min) in serum-free DMEM
    Lane 3 : PC12 cell lysate - treated with PMA-100nM (30min) in serum-free DMEM
    Lane 4 : PC12 cell lysate treated with EGF-100ng/ml (5min) with 10% FBS in DMEM

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 41, 43 kDa

  • Immunocytochemical analysis of HEK293 cells labelling phospho-ERK1 + ERK2 with ab136926 at 1/100 dilution.
  • Immunocytochemical analysis of HEK293 cells (hydrogen peroxide treated) labelling phospho-ERK1 + ERK2 with ab136926 at 1/100 dilution.

References

This product has been referenced in:

  • He X  et al. HOXB7 overexpression promotes cell proliferation and correlates with poor prognosis in gastric cancer patients by inducing expression of both AKT and MARKs. Oncotarget 8:1247-1261 (2017). WB ; Human . Read more (PubMed: 27901487) »
  • Venegas LA  et al. A comparison of phosphospecific affinity reagents reveals the utility of recombinant Forkhead-associated domains in recognizing phosphothreonine-containing peptides. N Biotechnol 33:537-43 (2016). Read more (PubMed: 26772725) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab136926.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up