Overview

  • Product name
  • Description
    Rabbit polyclonal to ERK2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Positive control
    • A431 cells, Breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab15282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
  • Application notes
    IHC-P: 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM
    citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
      Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • Domain
      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications
      Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ERK 2 antibody
      • ERK antibody
      • ERK-2 antibody
      • ERT1 antibody
      • Extracellular Signal Regulated Kinase 2 antibody
      • Extracellular signal-regulated kinase 2 antibody
      • MAP kinase 1 antibody
      • MAP kinase 2 antibody
      • MAP kinase isoform p42 antibody
      • MAPK 1 antibody
      • MAPK 2 antibody
      • Mapk1 antibody
      • MAPK2 antibody
      • Mitogen-activated protein kinase 1 antibody
      • Mitogen-activated protein kinase 2 antibody
      • MK01_HUMAN antibody
      • P38 antibody
      • P40 antibody
      • P41 antibody
      • p42-MAPK antibody
      • P42MAPK antibody
      • PRKM1 antibody
      • PRKM2 antibody
      • protein kinase, mitogen-activated, 1 antibody
      • protein kinase, mitogen-activated, 2 antibody
      • protein tyrosine kinase ERK2 antibody
      see all

    Images

    • ab15282, at 1/200 dilution, staining ERK2 in breast carcinoma by IHC-P.

    References

    This product has been referenced in:
    • Fu Y  et al. Functional ectopic neuritogenesis by retinal rod bipolar cells is regulated by miR-125b-5p during retinal remodeling in RCS rats. Sci Rep 7:1011 (2017). Read more (PubMed: 28432360) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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