Overview

  • Product name

  • Description

    Rabbit polyclonal to ERK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Dog, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment within Human ERK2 (internal sequence). The exact sequence is proprietary.
    Database link: P28482

  • Positive control

    • WB: HeLa cell extract; HeLa and H1299 whole cell lysates; Mouse brain lysate. IHC-P: Human colon carcinoma and breast carcinoma tissues. IP: HeLa whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab227134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/10000. Predicted molecular weight: 41 kDa.

Target

  • Function

    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ERK 2 antibody
    • ERK-2 antibody
    • ERT1 antibody
    • Extracellular Signal Regulated Kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • P38 antibody
    • P40 antibody
    • P41 antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • protein kinase, mitogen-activated, 1 antibody
    • protein kinase, mitogen-activated, 2 antibody
    • protein tyrosine kinase ERK2 antibody
    see all

Images

  • All lanes : Anti-ERK2 antibody (ab227134) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : ERK2 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 41 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded human colon carcinoma tissue stained for ERK2 using ab227134 at 1/500 dilution in immunohistochemical analysis.

  • ERK2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 4 µg ab227134. Western blot was performed from the immunoprecipitate using ab227134 at 1/500 dilution. Anti-rabbit IgG was used as a secondary reagent.

    Lane 1: HeLa whole cell extract 30 µg.

    Lane 2: Control IP in HeLa whole cell extract with 4 μg of preimmune Rabbit IgG.

    Lane 3: ab227134 IP in HeLa whole cell extract.

    10% SDS-PAGE.

  • All lanes : Anti-ERK2 antibody (ab227134) at 1/1000 dilution

    Lane 1 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 41 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded human breast carcinoma tissue stained for ERK2 using ab227134 at 1/500 dilution in immunohistochemical analysis.

  • Anti-ERK2 antibody (ab227134) at 1/5000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 41 kDa



    10% SDS-PAGE gel.

References

ab227134 has not yet been referenced specifically in any publications.

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