Overview

  • Product name

    Anti-ERK2 antibody [E460] (HRP)
    See all ERK2 primary antibodies
  • Description

    Rabbit monoclonal [E460] to ERK2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Immunogen

    Synthetic peptide within Human ERK2 aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P28482
    (Peptide available as ab205612)

  • Positive control

    • WB: HeLa, 46C, PC12 whole cell lysates and Human ERK2 recombinant protein lysate.
  • General notes

    Alternative versions available:
    Anti-ERK2 antibody [E460] (ab32081) - Knockout validated

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab205819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

  • Function

    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ERK 2 antibody
    • ERK-2 antibody
    • ERT1 antibody
    • Extracellular Signal Regulated Kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • P38 antibody
    • P40 antibody
    • P41 antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • protein kinase, mitogen-activated, 1 antibody
    • protein kinase, mitogen-activated, 2 antibody
    • protein tyrosine kinase ERK2 antibody
    see all

Images

  • All lanes : Anti-ERK2 antibody [E460] (HRP) (ab205819) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate (ab150035) at 10 µg
    Lane 2 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate at 10 µg
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
    Lane 4 : Recombinant Human ERK1 protein (ab43623) (ab43623) at 0.1 µg
    Lane 5 : Recombinant Human ERK2 protein (ab43625) at 0.1 µg
    Lane 6 : Wild-type HAP1 cell lysate at 20 µg
    Lane 7 : ERK2 knockout HAP1 cell lysate at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 12 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205819 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab205819 has not yet been referenced specifically in any publications.

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