Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E460] to ERK2 (HRP)
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Conjugation: HRP
Product nameAnti-ERK2 antibody [E460] (HRP)
See all ERK2 primary antibodies
DescriptionRabbit monoclonal [E460] to ERK2 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Recombinant fragment
- WB: HeLa, 46C, PC12 whole cell lysates and Human ERK2 recombinant protein lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab205819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
- Information by UniProt
- ERK 2 antibody
- ERK-2 antibody
- ERT1 antibody
All lanes : Anti-ERK2 antibody [E460] (HRP) (ab205819) at 1/5000 dilution
Lane 1 :
HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate at 10 µg
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 4 : Recombinant Human ERK1 protein (ab43623) (ab43623) at 0.1 µg
Lane 5 :
Recombinant Human ERK2 protein (ab43625) at 0.1 µg
Lane 6 : Wild-type HAP1 cell lysate at 20 µg
Lane 7 : ERK2 knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 12 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205819 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab205819 has not yet been referenced specifically in any publications.