Overview

  • Product name

  • Description

    Rabbit polyclonal to ERK5
  • Host species

    Rabbit
  • Specificity

    ab196609 detects endogenous level of total ERK5 protein.
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ERK5 (N terminal). The exact sequence is proprietary.
    Database link: Q13164

  • Positive control

    • HeLa, K562, romas, and 293T cell lysates. Mouse brain and lung tissue lysates. Paraffin-embedded Human esophagus cancer tissue, paraffin-embedded rat kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab196609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 88 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways.
  • Tissue specificity

    Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates to the nucleus upon activation.
  • Information by UniProt
  • Database links

  • Alternative names

    • Big MAP kinase 1 antibody
    • BMK 1 antibody
    • BMK 1 kinase antibody
    • BMK-1 antibody
    • BMK1 antibody
    • BMK1 Kinase antibody
    • EC 2.7.11.24 antibody
    • ERK 4 antibody
    • ERK 5 antibody
    • ERK-5 antibody
    • ERK4 antibody
    • ERK5 antibody
    • Extracellular signal regulated kinase 5 antibody
    • Extracellular signal-regulated kinase 5 antibody
    • MAP kinase 7 antibody
    • MAPK 7 antibody
    • MAPK7 antibody
    • Mitogen activated protein kinase 7 antibody
    • Mitogen-activated protein kinase 7 antibody
    • MK07_HUMAN antibody
    • OTTHUMP00000065906 antibody
    • OTTHUMP00000065907 antibody
    • PRKM 7 antibody
    • PRKM7 antibody
    • PROTEIN KINASE, MITOGEN-ACTIVATED, 7 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling ERK5 using ab196609 at 1/100 dilution.

  • Anti-ERK5 antibody (ab196609) at 1/250 dilution + Human fetal muscle tissue at 40 µg

    Secondary
    Goat anti rabbit IgG at 1/8000 dilution

    Predicted band size: 88 kDa


    Exposure time: 40 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human liver cancer tissue using ab196609 at dilution 1/20, the image on the right is treated with fusion protein.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human lung cancer tissue using ab196609 at dilution 1/20, the image on the right is treated with fusion protein.

  • Immunohistochemical analysis of paraffin-embedded Human esophagus labeling ERK5 using ab196609 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded rat kidney labeling ERK5 using ab196609 at 1/100 dilution.

  • Immunofluorescence analysis of MCF7 cells labeling ERK5 usingab196609 at 1/50 dilution. Blue: DAPI for nuclear staining.

References

This product has been referenced in:

  • Shi KL  et al. Atorvastatin antagonizes the visfatin-induced expression of inflammatory mediators via the upregulation of NF-?B activation in HCAECs. Oncol Lett 12:1438-1444 (2016). Human . Read more (PubMed: 27446449) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon adenocarcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 8.5
Permeabilization
No
Specification
Colon adenocarcinoma
Fixative
Formaldehyde

Mr. Allan Wang

Verified customer

Submitted Mar 20 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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