Key features and details
- Rabbit polyclonal to ERK8 (phospho T175 + Y177)
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ERK8 (phospho T175 + Y177) antibody
See all ERK8 primary antibodies
DescriptionRabbit polyclonal to ERK8 (phospho T175 + Y177)
ab73209 detects endogenous levels of ERK8 only when phosphorylated at threonine 175 and tyrosine 177 (Human) or threonine 176 and tyrosine 178 (Mouse).
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- Jurkat cell extract.
Previously labelled as MAPK15.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab73209 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab73209 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa).|
FunctionIn vitro, phosphorylates MBP.
Tissue specificityWidely expressed with a maximal expression in lung and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.
Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.
- Information by UniProt
- ERK 7 antibody
- ERK 8 antibody
- ERK-7 antibody
All lanes : Anti-ERK8 (phospho T175 + Y177) antibody (ab73209) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
ab73209 has been referenced in 1 publication.
- Jin DH et al. Overexpression of MAPK15 in gastric cancer is associated with copy number gain and contributes to the stability of c-Jun. Oncotarget 6:20190-203 (2015). WB . PubMed: 26035356