Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8088] to Erlin-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Product nameAnti-Erlin-2 antibody [EPR8088]
See all Erlin-2 primary antibodies
DescriptionRabbit monoclonal [EPR8088] to Erlin-2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Erlin-2 aa 50-150. The exact sequence is proprietary.
- Fetal kidney, 293T, HepG2, and HeLa lysates; Human endometrium adenocarcinoma; HepG2 cells
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as SPFH2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.40 x 10 -13 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 38 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionComponent of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway.
Sequence similaritiesBelongs to the band 7/mec-2 family.
Cellular localizationEndoplasmic reticulum membrane. Associated with lipid raft-like domains of the endoplasmic reticulum membrane.
- Information by UniProt
- C8orf2 antibody
- Chromosome 8 open reading frame 2 antibody
- Endoplasmic reticulum lipid raft associated protein 2 antibody
All lanes : Anti-Erlin-2 antibody [EPR8088] (ab129207) at 1/1000 dilution
Lane 1 : Fetal kidney lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 38 kDa
ab129207, at 1/50 dilution, staining Erlin-2 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab129207, at 1/100 dilution, staining Erlin-2 in HepG2 cells by Immunofluorescence.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129207 has not yet been referenced specifically in any publications.