Overview

  • Product name

    Anti-Erlin-2 antibody [EPR8089]
    See all Erlin-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8089] to Erlin-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Erlin-2 aa 300-400 (C terminal). The exact sequence is proprietary.
    Database link: O94905

  • Positive control

    • Fetal kidney, 293T and HepG2 lysates. Human colon tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as SPFH2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the band 7/mec-2 family.
    • Cellular localization

      Endoplasmic reticulum membrane. Associated with lipid raft-like domains of the endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • C8orf2 antibody
      • Chromosome 8 open reading frame 2 antibody
      • Endoplasmic reticulum lipid raft associated protein 2 antibody
      • Endoplasmic reticulum lipid raft-associated protein 2 antibody
      • ER lipid raft associated 2 antibody
      • ERLIN 2 antibody
      • Erlin-2 antibody
      • ERLIN2 antibody
      • ERLN2_HUMAN antibody
      • HGNC:1356 antibody
      • MGC87072 antibody
      • NET32 antibody
      • SPFH 2 antibody
      • SPFH domain containing protein 2 antibody
      • SPFH domain family member 2 antibody
      • SPFH domain-containing protein 2 antibody
      • SPFH2 antibody
      • SPG18 antibody
      • Stomatin prohibitin flotillin HflC/K domain containing protein 2 antibody
      • Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2 antibody
      see all

    Images

    • All lanes : Anti-Erlin-2 antibody [EPR8089] (ab128924) at 1/10000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 38 kDa

    • ab128924 at 1/50 dilution staining Erlin-2 in paraffin-embedded Human colon tissue by immunohistochemistry.

    References

    ab128924 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Gel Running Conditions
    Reduced Denaturing (8% Bis-Tris gel, MOPS buffer)
    Loading amount
    20 µg
    Specification
    HEK293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Feb 27 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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