Key features and details
- Goat polyclonal to ERO1L
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ERO1L antibody
See all ERO1L primary antibodies
DescriptionGoat polyclonal to ERO1L
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
- WB: A431, HeLa and Jurkat cell lysate. IHC-P: Human small intestine tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223646 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionEssential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
Tissue specificityWidely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
Sequence similaritiesBelongs to the EROs family.
The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397.
Cellular localizationEndoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.
- Information by UniProt
- Endoplasmic oxidoreductin 1 like protein antibody
- Endoplasmic oxidoreductin-1-like protein antibody
- Endoplasmic reticulum oxidoreductase 1 alpha antibody
Anti-ERO1L antibody (ab223646) at 0.3 µg/ml + A431 (human epidermoid carcinoma cell line) cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
The observed molecular weight corresponds to the glycosylated form.
ab223646 has not yet been referenced specifically in any publications.