Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12474] to ERO1L
- Suitable for: WB, IHC-P, Flow Cyt, IP
- Reacts with: Mouse, Human
Product nameAnti-ERO1L antibody [EPR12474]
See all ERO1L primary antibodies
DescriptionRabbit monoclonal [EPR12474] to ERO1L
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human ERO1L aa 1-100. The exact sequence is proprietary.
Database link: Q96HE7
- HepG2, 293T, MCF7 and HeLa whole cell lysate (ab150035). Human colon tissue. Permeabilized HeLa cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
FunctionEssential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
Tissue specificityWidely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
Sequence similaritiesBelongs to the EROs family.
The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397.
Cellular localizationEndoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.
- Information by UniProt
- Endoplasmic oxidoreductin 1 like protein antibody
- Endoplasmic oxidoreductin-1-like protein antibody
- Endoplasmic reticulum oxidoreductase 1 alpha antibody
All lanes : Anti-ERO1L antibody [EPR12474] (ab177156) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ERO1L with ab177156 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cell lysate or (Lane 2) 1 X PBS (negative control) using ab177156 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized HeLa cells labeling ERO1L with ab177156 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
ab177156 has been referenced in 1 publication.
- Takei N et al. Hypoxia-inducible ERO1a promotes cancer progression through modulation of integrin-ß1 modification and signalling in HCT116 colorectal cancer cells. Sci Rep 7:9389 (2017). PubMed: 28839225