Overview

  • Product name

    Anti-ERO1L antibody [EPR12474]
    See all ERO1L primary antibodies
  • Description

    Rabbit monoclonal [EPR12474] to ERO1L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ERO1L aa 1-100. The exact sequence is proprietary.
    Database link: Q96HE7

  • Positive control

    • HepG2, 293T, MCF7 and HeLa whole cell lysate (ab150035). Human colon tissue. Permeabilized HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12474
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10 - 1/100.

Target

  • Function

    Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
  • Tissue specificity

    Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
  • Sequence similarities

    Belongs to the EROs family.
  • Post-translational
    modifications

    N-glycosylated.
    The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397.
  • Cellular localization

    Endoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • Endoplasmic oxidoreductin 1 like protein antibody
    • Endoplasmic oxidoreductin-1-like protein antibody
    • Endoplasmic reticulum oxidoreductase 1 alpha antibody
    • Endoplasmic reticulum oxidoreductin 1-like antibody
    • ERO1 alpha antibody
    • ERO1 L antibody
    • ERO1 Lalpha antibody
    • ERO1 like protein alpha antibody
    • ERO1-alpha antibody
    • ERO1-L antibody
    • ERO1-L-alpha antibody
    • ERO1-like (S. cerevisiae) antibody
    • ERO1-like alpha antibody
    • ERO1-like protein alpha antibody
    • ERO1-like, S. cerevisiae, homolog of, alpha antibody
    • ERO1A antibody
    • ERO1A_HUMAN antibody
    • ERO1L antibody
    • ERO1LA antibody
    • Oxidoreductin 1 Lalpha antibody
    • Oxidoreductin-1-L-alpha antibody
    • PRO865 antibody
    • UNQ434 antibody
    see all

Images

  • All lanes : Anti-ERO1L antibody [EPR12474] (ab177156) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 54 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ERO1L with ab177156 at 1/100 dilution.

  • Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cell lysate or (Lane 2) 1 X PBS (negative control) using ab177156 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Flow cytometric analysis of permeabilized HeLa cells labeling ERO1L with ab177156 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

References

This product has been referenced in:

  • Takei N  et al. Hypoxia-inducible ERO1a promotes cancer progression through modulation of integrin-ß1 modification and signalling in HCT116 colorectal cancer cells. Sci Rep 7:9389 (2017). Read more (PubMed: 28839225) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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