Recombinant
RabMAb

Recombinant Anti-ERp18 antibody [EPR9024] (ab134938)

Submit a review Submit a question

Overview

  • Product name

    Anti-ERp18 antibody [EPR9024]
    See all ERp18 primary antibodies

  • Description

    Rabbit monoclonal [EPR9024] to ERp18

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, IP, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ERp18 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HepG2 cells; Human kidney tissue; Human placenta, 293T, HepG2 and Y79 lysates.

  • General notes

     

     

     This product was previously labelled as ERp19

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9024

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Possesses significant protein thiol-disulfide oxidase activity.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Contains 1 thioredoxin domain.

    • Cellular localization

      Endoplasmic reticulum lumen.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AG1 antibody
      • AGR1 antibody
      • anterior gradient homolog 1 antibody
      • endoplasmic reticulum protein ERp19 antibody
      • Endoplasmic reticulum resident protein 18 antibody
      • Endoplasmic reticulum resident protein 19 antibody
      • endoplasmic reticulum thioredoxin superfamily member, 18 kDa antibody
      • ER protein 18 antibody
      • ER protein 19 antibody
      • ERP 18 antibody
      • ERP16 antibody
      • ERp18 antibody
      • ERp19 antibody
      • hAG 1 antibody
      • hAG1 antibody
      • hTLP19 antibody
      • PDIA16 antibody
      • protein disulfide isomerase family A, member 16 antibody
      • thioredoxin domain containing 12 (endoplasmic reticulum antibody
      • Thioredoxin domain-containing protein 12 antibody
      • thioredoxin like protein p19 antibody
      • Thioredoxin-like protein p19 antibody
      • TLP19 antibody
      • TXD12_HUMAN antibody
      • TXNDC12 antibody
      see all

    Images

    • Western blot - Anti-ERp18 antibody [EPR9024] (ab134938)
      Western blot - Anti-ERp18 antibody [EPR9024] (ab134938)
      All lanes : Anti-ERp18 antibody [EPR9024] (ab134938) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Y79 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 19 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-ERp18 antibody [EPR9024] (ab134938)
      Immunocytochemistry/ Immunofluorescence - Anti-ERp18 antibody [EPR9024] (ab134938)

      Immunofluorescence analysis of HepG2 cells, labelling ERp18 using ab134938 at a 1/250 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp18 antibody [EPR9024] (ab134938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp18 antibody [EPR9024] (ab134938)

      Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human kidney tissue, labelling ERp18 using ab134938 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab134938 has not yet been referenced specifically in any publications.

    Publishing research using ab134938? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab134938.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com