Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9024] to ERp18
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-ERp18 antibody [EPR9024]
See all ERp18 primary antibodies
DescriptionRabbit monoclonal [EPR9024] to ERp18
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ERp18 aa 1-100. The exact sequence is proprietary.
- HepG2 cells; Human kidney tissue; Human placenta, 293T, HepG2 and Y79 lysates.
This product was previously labelled as ERp19
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.|
|ICC/IF||1/250 - 1/500.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 19 kDa.|
FunctionPossesses significant protein thiol-disulfide oxidase activity.
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 thioredoxin domain.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- AG1 antibody
- AGR1 antibody
- anterior gradient homolog 1 antibody
All lanes : Anti-ERp18 antibody [EPR9024] (ab134938) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Y79 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 19 kDa
Immunofluorescence analysis of HepG2 cells, labelling ERp18 using ab134938 at a 1/250 dilution.
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human kidney tissue, labelling ERp18 using ab134938 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab134938 has not yet been referenced specifically in any publications.