Product nameAnti-ERp18 antibody [EPR9024]
See all ERp18 primary antibodies
DescriptionRabbit monoclonal [EPR9024] to ERp18
Tested applicationsSuitable for: IHC-P, ICC/IF, IP, WBmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ERp18 aa 1-100. The exact sequence is proprietary.
- HepG2 cells; Human kidney tissue; Human placenta, 293T, HepG2 and Y79 lysates.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.|
|ICC/IF||1/250 - 1/500.|
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 19 kDa.|
FunctionPossesses significant protein thiol-disulfide oxidase activity.
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 thioredoxin domain.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- AG1 antibody
- AGR1 antibody
- anterior gradient homolog 1 antibody
All lanes : Anti-ERp18 antibody [EPR9024] (ab134938) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Y79 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 19 kDa
Immunofluorescence analysis of HepG2 cells, labelling ERp18 using ab134938 at a 1/250 dilution.
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human kidney tissue, labelling ERp18 using ab134938 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab134938 has not yet been referenced specifically in any publications.